Nuclear localization signal-IN (NLS(IN))

Description

Product name: Nuclear localization signal-IN (NLS(IN))

Catalog#: 1809006

Synonyms: HIV-1 Integrase (161–173),

CAS NO.:

Sequence: IIGQVRDQAEHLK

M.W:1505.83

M.F.:C65H111N21O20

Purity: 95% by HPLC

Counter ion: Trifluoacetate

Format: Lyophilized powder

Description: In spite of recent efforts to elucidate the nuclear import pathway of the human immunodeficiency virus type 1 (HIV-1) integrase protein (IN), its exact route as well as the domains that mediate its import are still unknown. Here, we show that a synthetic peptide bearing the amino acid residues 161–173 of the HIV-1 IN is able to mediate active import of covalently attached bovine serum albumin molecules into nuclei of permeabilized cells and therefore was designated as nuclear localization signal-IN (NLS(IN)). A peptide bearing residues 161–173 in the reversed order showed low karyophilic properties. The NLS(IN) as well as the full-length IN protein interacted specifically with importin α, binding of which was blocked by the NLS(IN) peptide itself as well as by the NLS-SV40.

Uniprot ID:

 

Usage: For Scientific Research Use Only, Not for Human Use.