DBDY-Gly-Phe-MTPA

Description

Product name: DBDY-Gly-Phe-MTPA

Catalog#: 1602020

Synonyms: N,N’-diBoc-dityrosine-glycine-phenylalanine-3-(methylthio)propylamine,

CAS NO.:

Sequence: DBDY-Gly-Phe-MTPA

M.W: C43H57N5O11S

M.F.: 852.01

Purity: 95% by HPLC

Counter ion: Trifluoroacetate

Format: Lyophilized powder

Description: Cathepsin B has been suggested to be a prognostic marker of melanoma, glioma, and a variety of cancers such as brain, breast, colon, esophageal, gastric, lung, ovarian, and thyroid cancers. Cathepsin B inhibitors have also been considered as anticancer drug candidates; hence, there has been a growing need for a probe which enables the selective and simple detection of cathepsin B and its inhibitors. For the purpose of selective assay, a cathepsin B-specific substrate, N,N′-diBoc-dityrosine-glycine-phenylalanine-3-(methylthio)propylamine (DBDY-Gly-Phe-MTPA) was synthesized in this study. Phe-MTPA, which was produced via cathepsin B-catalyzed hydrolysis of DBDY-Gly-Phe-MTPA, allowed aggregation of gold nanoparticles (AuNPs) leading to a color change from red to blue. When tested for cathepsins B, L, and S, this assay method exhibited AuNPs color change only in reaction to cathepsin B. The colorimetric assay of cathepsin B and cathepsin B inhibitors using DBDY-Gly-Phe-MTPA and AuNPs allowed not only the selective but also the simple assay of cathepsin B and its inhibitors, which was possible with the naked eye.

Compound ID:

 

Usage: For Scientific Research Use Only, Not for Human Use.