PepFect 14

$ 540.0

Quantity: 10 MG

In stock (can be backordered)


Product name: PepFect 14(PF14)

Catalog#: 1101012

Synonyms:PF14, PepFect14, PepFect-14, Stearyl-AGYLLGKLLOOLAAAALOOLL

Sequence: Stearyl-Ala-Gly-Tyr-Leu-Leu-Gly-Lys-Leu-Leu-Orn-Orn-Leu-Ala-Ala-Ala-Ala-Leu-Orn-Orn-Leu-Leu-NH2

Modifications: N-terminal Stearic Acid, C-terminal amide, O, ornithine,

M.W: 2406.22

M.F.: C120H217N27O23

Purity: 95% by HPLC

Counter ion: Trifluoacetate

Format: Lyophilized powder

Description: PepFect 14, a novel cell-penetrating peptide for oligonucleotide delivery in solution and as solid formulation. PepFect 14 (PF14) is a modified version of our previously reported peptide, stearyl-transportan10 (stearyl-TP10), where we utilized ornithines and leucines instead of lysines and isoleucines. PepFect 14 (PF14), forms non-covalent nanocomplexes with short interfering RNA (siRNA), which are able to elicit efficient RNA-interference (RNAi) response in different cell-lines. RNAi effect is obtained at low siRNA doses with a unique kinetic profile. Furthermore, the solid dispersion technique is utilized to formulate PF14/siRNA nanocomplexes into solid formulations that are as active as the freshly prepared nanocomplexes in solution. Importantly, the nanocomplexes are stable and active in mediating RNAi response after incubation with simulated gastric fluid (SGF) that is highly acidic. These results demonstrate the activity of PF14 in delivering and protecting siRNA in different pharmaceutical forms and biological environments. PF14 demonstrates remarkable splice-correction activity in two different cell models including mdx mouse myotubes, a cell culture model of DMD, even in the presence of serum. Furthermore, the feasibility of formulating these nanocomplexes into solid formulation was investigated and the efficiency and stability of such formulations were assessed. The amphipathic, N-stearylated CPP, PF14 forms aggregates with heterogeneous shape and polydisperse size.