Quantity: 50 MG
Available on backorder
Product name: Strep-tag affinity peptide
Purity: 95% by HPLC
Counter ion: Trifluoacetate
Format: Lyophilized powder
Description: The Strep-tag is a selected nine-amino acid peptide (AWRHPQFGG) that displays intrinsic binding affinity towards streptavidin and has been used as an affinity tag for recombinant proteins. Hence, a corresponding fusion protein can be eluted under gentle buffer conditions—which may be optimized for a given polypeptide and can even contain additives like thiol compounds or detergents—from an affinity column with immobilized streptavidin, just by applying a low concentration of biotin or related compounds as competitor . This method usually permits the efficient purification of a recombinant protein in a single step. Another advantage of the Strep-tag is its independence from metal ions in the purification, an aspect often of interest when metallo-proteins are to be studied or when paramagnetic impurities must be avoided for NMR.
Usage: For Scientific Research Use Only, Not for Human Use.