$ 280.0
Quantity: 10Gram
In stock
Description
Product name: AB-NTA, hydrate
Synonym: NA,NA-BIS(CARBOXYMETHYL)-L-LYSINE HYDRATE; NAlpha,NAlpha-Bis(carboxymethyl)-L-lysine hydrate;
Catalog #: 8604039-1
Purity: 97%
CAS No.: 941689-36-7
Molecular Formula: C10H20N2O7
Molecular Weight: 280.275
Compound ID: 7019830
Description: Nitrilotriacetic acid has been routinely used in protein purification for its high affinity for His-tagged protein in the presence of Ni2+. Superparamagnetic Fe3O4/Au nanoparticles were synthesized and surface modified with mercaptopropionic acid (MPA), followed by conjugating Nα,Nα-Bis(carboxymethyl)-l-lysine hydrate (ANTA) and subsequently chelating Co(2+). The resulting Fe3O4/Au-ANTA-Co(2+) nanoparticles have an average size of 210 nm in aqueous solution, and a magnetization of 36 emu/g, endowing the magnetic nanoparticles with excellent magnetic responsivity and dispersity. The Co(2+) ions in the magnetic nanoparticle shell provide docking site for histidine, and the Fe3O4/Au-ANTA-Co(2+) nanoparticles exhibit excellent performance in binding of a His-tagged protein with a binding capacity of 74 μg/mg. The magnetic nanoparticles show highly selective purification of the His-tagged protein from Escherichia coli lysate. Therefore, the obtained Fe3O4/Au-ANTA-Co(2+) nanoparticles exhibited excellent performance in the direct separation of His-tagged protein from cell lysate. A building block based on a tailored metal-chelating amino acid. Nepsilon,Nepsilon-bis(carboxymethyl)-L-lysine can be flexibly introduced into peptides by 9-fluorenylmethoxycarbonyl solid-phase chemistry. The corresponding metal-chelating peptides act as metal sensors and synthetic receptors for histidine-tagged proteins.
