Quantity: 10 MG
Product name: PepFect 3
Synonyms: PF3, PepFect 3, stearyl-TP10, Stearyl-AGYLLGKINLKALAALAKKIL-NH2,
Modifications: N-terminal Stearic Acid ,C-terminal amide,
Purity: 95% by HPLC
Counter ion: Trifluoacetate
Format: Lyophilized powder
Description: PF3 is N‐terminally stearylated TP10. It is known that acylation of peptides by fatty acid might increase the ability of peptides for cell internalization; some acylated peptides were able to penetrate cell membrane and enter the cells even if this was not the case for their non-acylated analogs. However, stearylation cannot be regarded as a generally beneficent modification leading to the improved cell-penetration of peptides, since it was shown that it did not increase cell-penetrating efficiency of CPPs penetratin and nona-arginine. In the case of TP10, stearylation leading to PF3 results in substantial increase of the uptake of CPP‐oligonucleotide complexes and, as a consequence, splice‐correction. The effectiveness of transfection was found to be comparable to that for cationic lipid Lipofectamine 2000™.